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  • MitoSceneTM Green I(線粒體綠色熒光探針)

    產(chǎn)品貨號(hào): M4063S/M4063L

    產(chǎn)品規(guī)格: 50 μg / 20 x 50 μg

    目錄價(jià)(元):350/3200

    推薦儀器:熒光顯微鏡、流式細(xì)胞儀、熒光酶標(biāo)儀

    大包裝詢價(jià)


產(chǎn)品概述:

產(chǎn)品參數(shù)
外觀:可溶于 DMSO、DMF 的紅色固體
Ex/Em:490/523 nm(in MeOH)
CAS 號(hào):201860-17-5

分子式:C34H28Cl5N3O
分子量:671.9
分子結(jié)構(gòu)圖:



儲(chǔ)存條件
-20℃避光保存,有效期見外包裝。

產(chǎn)品介紹
MitoSceneTM Green I 是一種線粒體綠色熒光染料,可在納摩爾水平對(duì)線粒體進(jìn)行染色標(biāo)記,該染料具有質(zhì)膜透性,且在線粒體膜上積累而呈現(xiàn)明亮的熒光。該染料在線粒體中的定位不依賴于線粒體膜電位,染色固定細(xì)胞信噪比不理想,在細(xì)胞固定、透化之后,會(huì)導(dǎo)致熒光信號(hào)減弱或丟失,所以我們推薦只用來(lái)染活細(xì)胞。

注意事項(xiàng)
1. 熒光染料均存在淬滅問(wèn)題,請(qǐng)盡量注意避光,以減緩熒光淬滅。
2. 為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

說(shuō)明書:


 M4063S/M4063L    

使用本產(chǎn)品的文獻(xiàn):


1.Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells
PengYuana,TaoYang,JiaoMu,JingZhao,YiYang,ZhaoyongYan,YiranHou,ChengChen,JinliangXing,HongxinZhang,JibinLi

Cancer Letters Volume 469, 28 January 2020, Pages 498-509

2. Transcriptional characteristics and functional validation of three monocyte subsets during aging.

Chen Wang, Yating Cheng , Boyu Li , Xueping Qiu , Hui Hu , Xiaokang Zhang , Zhibing Lu and Fang Zheng

Wang et al. Immunity & Ageing (2023) 20:50



參考文獻(xiàn)
1.Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs.
應(yīng)用方向:分析HL60細(xì)胞線粒體膜電位

2.Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissUEs.
應(yīng)用方向:對(duì)線粒體特異染色,線粒體形態(tài)和功能的研究

3.MitoTracker Green labeling of mitochondrial proteins and their subseqUEnt analysis by capillary electrophoresis with laser-induced fluorescence detection.
應(yīng)用方向:標(biāo)記線粒體是通過(guò)與線粒體的蛋白特異結(jié)合

4.Mitotracker green is a P-glycoprotein substrate.
應(yīng)用方向:染料是一種P-糖蛋白底物

5.Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells.
應(yīng)用方向:追蹤16HBE氣道上皮細(xì)胞的有絲分裂

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